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Nowadays, available phosphorus (P) deficiency in soil and weed resistance to herbicides have emerged as two severe limiting factors for sustainable agriculture. Therefore, it is of urgent needs to improve plant absorption/utilization ability of the soil P, seek phosphate (Pi)-alternative P fertilizers, and develop new forms of weed control systems. Phosphite (Phi), as a P resource of relatively high amount only less than Pi in Earth, can be converted to utilizable Pi uniquely in some bacterial species by oxidization via its specific dehydrogenase (PTDH), but inhibits plant growth and development. This implies that Phi might rather become a suitable P fertilizer for plants if introducing a PTDH detoxifier from bacteria. Herein, we created the transgenic tobaccos harboring a Pseudomonas PTDH gene (PsPtx) amplified from the soil metagenome previously. RT-PCR showed that the exotic PsPtx gene could express similarly in root, stem and leaf tissues of all transgenic lines. PsPtx transgenic tobaccos could utilize Phi by oxidization as the sole Pi supply, and also outperformed wild-type tobacco with a remarkably dominant growth under Phi stress conditions. Moreover, the PsPtx gene was preliminarily evaluated with a notable quality as a potential candidate of the selection marker in plant genetic transformation. Conclusively, PsPtx and its encoded phosphite dehydrogenase might be applicable for developing a dual system of plant phosphorus utilization and weed control using Phi as P fertilizer and herbicide, and provide an effectual solution to some obstacles in the current crop transgenic studies.
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Oxidoreductases , Phosphites , Phosphorus , Plants, Genetically Modified , Weed ControlABSTRACT
Objective To study and analyze the clinical features of pulmonary tuberculosis complicated with diabetes,and the risk factors affecting the prognosis of pulmonary tuberculosis patients complicated with diabetes by multivariate analysis.Methods From January 2012 to January 2015,200 tuberculosis patients complicated with diabetes in the Second Hospital of Yuncheng were selected in the research.The clinical data of 200 patients were retrospectively analyzed,the clinical characteristics of pulmonary tuberculosis with diabetes (age,gender,lung cavity etc) were summarized,the 2-year follow-up data were analyzed to analyze the recurrence.The patients were divid-ed into good prognosis group and poor prognosis group.The clinical data were compared between the two groups,multi-variate analysis and logistics regression analysis were used to analyze the risk factors affecting prognosis of pulmonary tuberculosis patients with diabetes.Results The distribution of pulmonary tuberculosis patients with diabetes in males was higher than females (P＜0.05),the age distribution was mainly over 60 years old,the incidence rate of lung cavity was 67.50%,the positive rate of sputum was 64.00%.After 2 years of follow-up,there were 42 cases of recurrence.Univariate analysis results showed that there were statistically significant differences in age,blood glucose control effect,drug resistance,drug compliance and other aspects between the poor prognosis group and the good prog -nosis group (all P＜0.05),and there were no statistically significant differences in gender,lung cavities,sputum posi-tive aspects between the two groups (all P＞0.05).The logistics linear regression analysis showed that the effect of blood glucose control,drug resistance and compliance were the risk factors affecting prognosis of pulmonary tuberculosis patients with diabetes.Conclusion Pulmonary tuberculosis complicated with diabetes occurred in elderly male popu-lation,the prognosis is often affected by effect of blood glucose control,drug resistance,medication compliance and other factors,clinical intervention should be carried out according to the prognostic risk factors.
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AIM: To investigate the effect of nucleolin on diabetic cardiomyopathy in mice.METHODS: A type II diabetic cardiomyopathy mouse model was prepared using a cardiac-specific nucleolin-overexpressing transgenic mice.The mice were divided into wild-type mouse control group, nucleolin transgenic mouse control group, wild-type mouse diabetes group and nucleolin transgenic mouse diabetes group.Wheat germ agglutinin (WGA) fluorescent dye, Masson staining and PowerLab system detection were used to further clarify the role of nucleolin on cardiac hypertrophy, fibrosis and cardiac function in type II diabetic cardiomyopathy mice.RESULTS: Compared with wild-type mouse control group, no significant increase in blood glucose level was found, while genetical myocardial cell hypertrophy was significantly attenuated in nucleolin transgenic mouse diabetes group.The collagen fibers were also significantly reduced, and hemodynamic indexes ± dp/dtmax, left ventricular end-diastolic pressure, left ventricular systolic pressure and heart rate were also improved.The above differences were statistically significant.CONCLUSION: Nucleolin may reduce the occurrence of myocardial hypertrophy and fibrosis, thus improving the cardiac function of diabetic cardiomyopathy mice.
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OBJECTIVE:To explore the effects of umbilical therapy by Rheum plamatum combined with early colonic irriga-tion on the quality of life in elderly patients with colon stoma. METHODS:80 patients underwent radical resection of abdominoperi-neal rectal carcinoma were randomly divided into control group and observation,with 40 cases in each group. Control group was given early colonic irrigation;observation group was additionally given umbilical therapy by R. plamatum. Treatment course of 2 groups lasted for one month,and both were followed up for 6 months after treatment. The quality by life in 2 groups was evaluated by using QLQ-C30,and therapy costs were calculated before and after treatment. RESULTS:Compared with before treatment, function score and total health score of 2 groups improved significantly in 1,3,6 months after treatment,while symptom score and single item score decreased significantly,with statistical significance(P<0.05). In 1,3,6 months after treatment,role func-tion,social function,cognitive function,emotional function and total health score of observation group were significantly higher than those of control group,and insomnia and pain score were significantly lower than control group,with statistical significance (P<0.05). 3 and 6 months after treatment,diarrhea and economic difficulty score of observation group were significantly lower than those of control group,with statistical significance(P<0.05). 1 months after treatment,loss of appetite score of observation group were significantly lower than those of control group,with statistical significance (P<0.05). 6 months after treatment, fa-tigue,nausea and vomiting score of observation group were significantly lower than those of control group,with statistical signifi-cance (P<0.05). 6 months after treatment,nursing cost,drug cost and total cost of observation group were significantly lower than those of control group,with statistical significance(P<0.05). CONCLUSIONS:The umbilical therapy by R. plamatum com-bined with early colonic irrigation can improve the quality of life in elderly patients with colon stoma significantly and reduce treat-ment cost.
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OBJECTIVE@#To investigate the nucleolus expression in the diabetic cardiomyopathy.@*METHODS@#The rats were divided into a control group and a type II diabetic cardiomyopathy group (model group). In the model group, rats were fed with high-fat and high-sugar food (rats were intravenously injected with 60 mg/kg chain urea with cephalosporins in the 5th and 6th weeks in mice). The level of blood glucose was determined at the end of 8th week and the level of fasting blood glucose was examined at the end of 20th week. The ratio of the heart mass and body mass was calculated, and the pathological changes in myocardial morphology were observed. The immunohistochemical method and Western blot were used to detect the expression level of myocardial nucleolin.@*RESULTS@#The level of fasting blood glucose was significantly increased in the diabetic model group than that in the control group (P<0.05). Rats in the model group were found hypertrophic cardic cells, with fracture, dissolusion, and disordered arrangement. Immunohistochemical staining and Western blot showed the protein levels of myocardial nucleolin in the model group were obviously higher than those in the control group (P<0.05).@*CONCLUSION@#Nucleolin may play a role in the pathogenesis and development of the diabetic cardiomyopathy.
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Animals , Rats , Blood Glucose , Diabetes Mellitus, Experimental , Metabolism , Diabetic Cardiomyopathies , Metabolism , Myocardium , Pathology , Phosphoproteins , Metabolism , RNA-Binding Proteins , MetabolismABSTRACT
AIM:To construct myocardium-specific nucleolin ( Ncl) transgenic mice and to provide an animal model for the studies of the myocardial protection of nucleolin .METHODS:To create nucleolin transgenic mice , a myo-cardium-specific expression plasmid of nucleolin ( Alpha-MyHC clone 26-Ncl) was constructed .The gene type of transgenic mice was identified by PCR and the nucleolin protein level was tested by Western blotting .The myocardium morphology , heart weight index (HWI) and left ventricular pressure maximum rise rate were observed in nucleolin transgenic (TG) mice and wild-type ( WT) mice.RESULTS:We gained 4 transgenic mice (51, 52, 56 and 86 lines, only 52 line and 86 line were eugonic) by PCR.Western blotting analysis showed the expression of nucleolin up-regulated specifically in the myocardium .However , the myocardium morphology , HWI and left ventricular pressure maximum rise rate in the nucleolin transgenic mice were similar to those in the wild-type mice.CONCLUSION:We constructed myocardium-specific nucleo-lin transgenic mice successfully .
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To study the effects of expressions of SCCA1 and SCCA2 in cervical squamous cell carcinoma on its diagnosis, treatment evaluation and prognosis analysis. Seventy-six cervical squamous cell carcinoma patients enrolled in our hospital from October 2011 to April 2013 were selected, and another 76 healthy females [without cervical tissue lesions] were enrolled as the control. SCCA1 and SCCA2 expressions in the two groups were compared by RT-PCR. The serodiagnosis results before and after chemotherapy were compared to clarify the effects of SCCA2 expression. The two groups had similar relative SCCA1 expression rates that were not significantly correlated with pathological factors. Before chemotherapy, the relative expression rates of SCCA2 were significantly higher in the patients with later stage [t=6.018, P=0.00082 < 0.05] and lymphatic metastasis [t=6.281, P=0.00192 < 0.05]. After treatment, relative SCCA2 expression rate was decreased more significantly in the effective group than that in the ineffective group [t=10.27893, P=0.02815 < 0.05]. The expression of SCCA1 failed to indicate the onset, diagnosis and prevention of cervical squamous cell carcinoma, whereas that of SCCA2 worked as one of the tumor markers
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OBJECTIVE To compare two different observed data from Nov 2003 to Apr 2004 and from Jan 2005 to Dec 2005 on hospital-acquired infection in our hospital and put forward the counterned measures.METHODS The bacteria samples from clinic were cultured.RESULTS The Pseudomonas aerugonosa,Acinetobacter baumannii and Escherichia coli were common strains of bacteria according to the two years data in our hospital.They were 30.35%,18.41% and 8.46% vs 19.74%,17.11% and 14.04%,respectively.The P.aerugonosa infective constituent ratio was decreased(30.35% vs 19.74%,P
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Objective To study the pharmacokinetics of ferulic acid in rabbits in vivo after ig administering Ruxian Pill.Methods The concentrations of ferulic acid in plasma were determined by HPLC.Taking coumarin as internal standard,a mixture of methanol-acetic acid-water(38.0∶0.5∶61.5) was used as the mobile phase,and UV detection was performed at 320 nm.The analytical column was ODS Hypersil C_(18)(150 mm?4.6 mm,5 ?m).Results The calibration curve was linear over the range of 12.5—1 600.0 ?g/L and the measurable limit of plasma detection was 6.25 ?g/L.The recovery was 96.17%,99.80%,100.23%. The RSD of the intra-day and the inter-days was less than 6%.The plasma concentration-time curve was consistent with one-comparment model.The pharmacokinetic parameters were as follows: AUC_(0-4 h)(855.29?100.78) ?g/(L?h),AUC_(0-∞)(1 051.99?125.96) ?g/(L?h),C_(max)=(952.71?119.04) ?g/(L?h),t_(max)=(0.5?0.00) h,t_(1/2)=(1.61?0.21) h,K_e=(0.46?0.05)/h. Conclusion The method is accurate and precise with better reproducibility,which can be used for the determination of plasma level of ferulic acid and the study of its pharmacokinetics for Ruxian Pill.
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Objective:To study on effect of Jiantai Liquid on progesterone receptor(PR)in the uterus at the nidation stage in the mouse with disturbance of blastocyst nidation.Methods:The gestational mice were randomly divided into a normal group,a model group and a Chinese drug group.Mifepristone was used for inducing disturbance of blastocyst nidation in the mice,which were interfered with Jiantai Liquid.At the eighth week of pregnancy,the mice were killed and morphological changes of the endometrium were observed,and PR expression in the uterus was detected with immunohistochemical method and PR and PRmRNA expressions were detected with Western blot and PT-PCR methods.Results:The endometrium showed maldevelopment in the model group and normal development in the Chinese drug group,similar to the normal group;the gland,the PR expression range and intensity of PR expression in the interstitial substance in the Chinese drug group increased as compared with the model group(P0.05);expressions of PR protein and gene in the Chinese drug group were significantly higher than those in the model group(P0.05).Conclusion:Jiantai Liquid can promote development of the endometrium and improves blastocyst nidation via regulating expressions of PR and PRmRNA in the uterus in the mice with disturbance of blastocyst nidation.
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Objective: To investigate the clinical effect of Huangqishengmai Oral Solution for Viral myocarditis. Methods: 128 patients in the therapeutic group were treated with Huangqishengmai Oral Solution. 72 patients in the control group were treated with routine treatment. The changes of heart rate, heart function, electrocar-diogram ST-T, arrhythmia, SOD and MDA before and after treatment were observed. Results: (a) The heart rates of both groups slowed down after treatment, and there were obvious differences in various markers of heart function between before and after treatment. (b) ST-T change of patients in the therapeutic group was obvious. The effective rate of the therapeutic group was higher than that of the control group. (c) SOD level of patients in the therapeutical group rised. MDA level decreased. (d) No obvious difference in treating arrhythmia was observed between two groups. Conclusion: Huangqishengmai Oral Solution is effective for viral myocarditis and should be widely applied in clinic.